Strain Data Sheet
RBRC12273
Strain Information | |
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| Image | |
| BRC No. | RBRC12273 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Runx3<tm3.1Itoy> |
| Former Common name | Runx3-R122C, Runx3-R122C/R122C or Runx3R122C/R122C |
| H-2 Haplotype | |
| ES Cell line | TT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1] |
| Background strain | |
| Appearance | |
| Strain development | 1. The Runx3 exon2-4 was isolated from C57BL/6 BAC clone using high-fidelity polymerase chain reaction.2. PCR-based Site-direct mutagenesis was performed to introduce the point mutation R122C in exon 3.3. The targeting vector was generated by cloning the genomic fragments containing the intronic region between exon 3 into the polylinker of the target vector NEO-DTA cassette contained a Pgk-Neor (neomycin resistant) gene flanked by LoxP sites and a DTA (diphtheria toxin) cassette for the selection.4. TT2 ES cells will be used for gene targeting.5. The neomycin expression cassette was then excised by crossing the chimera mice with EIIa-Cre mice. (Douchi et al., CMGH 2022)1. Targeted TT2 ES cell clone which has R123C mutation (equivalent of human RUNX3 R122C mutation) in exon 3 of Runx3 gene was injected into C57BL/6 embryos, which were then re-implanted into pseudo-pregnant females.2. Germline transmission was confirmed by genotyping of the offspring.3. Heterozygous targeted mice were generated and maintain strain by crossing with wild type C57BL/6 mice.4. This line was backcrossed to C57BL/6 for more than ten generations by the donating laboratory.5. This line is maintained as homozygote. |
| Strain description | Runx3 is a transcription factor and functions as a developmental regulator. Runx3 is frequently associated with cancer. R122C mutation (equivalent of mouse Runx3 R123C mutation) was originally identified in human gastric cancer. 30 % of Runx3-R122C/R122C homozygote mice show some precancerous metaplasia at 6-month age. The homozygote female mice can get pregnant normally. The homozygote mice can survive more than one year. |
| Colony maintenance | |
| References | Cell. Mol. Gastroenterol. Hepatol., 13(5) 1317-1345 (2022). 35074568 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Runx3 | runt related transcription factor 3 | 4 | Runx3 | targeted mutation 3.1, Yoshiaki Ito | |||
| loxP | phage P1 loxP | 4 | loxP | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Enterobacteria phage P1 LoxP site, mouse Runx3 genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Mol. Gastroenterol. Hepatol., 13(5) 1317-1345 (2022).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. A recipient shall acquire a prior written consent from the DEPOSITOR prior to filing an application for a patent, or intellectual property or other rights based on the results of research using the BIOLOGICAL RESOURCE. |
| Depositor | Yoshiaki Ito (National University of Singapore) |
| Strain Status |  Live mice |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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