Strain Data Sheet

RBRC12156

Strain Information

Image
BRC No.RBRC12156
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6;Cg-Oxtr<em4(3xHA/T2A/icre/ERT2)Yinn>/#8
Former Common nameOxtr<3xHA-iCreERT2>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Yukiko U. Inoue and Takayoshi Inoue, National Center of Neurology and Psychiatry in 2022. This strain was generated using fertilized eggs derived from (C57BL/6 x C3H)F1. Mixed genetic background.
Strain descriptionicre/ERT2 knockin mice via 3xHA/T2A at the C-terminus of the Oxtr gene. Homozygous mice are fertile.
Colony maintenance
References
Targeting Neurons with Functional Oxytocin Receptors: A Novel Set of Simple Knock-In Mouse Lines for Oxytocin Receptor Visualization and Manipulation.
Yukiko U Inoue, Hideki Miwa, Kei Hori, Ryosuke Kaneko, Yuki Morimoto, Eriko Koike, Junko Asami, Satoshi Kamijo, Mitsuhiko Yamada, Mikio Hoshino, Takayoshi Inoue
eNeuro, 9(1):ENEURO.0423-21.2022 (2022). 35082173

Health Report

Examination Date / Room / Rack2024/05/20Room:4-ARack:D

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2estrogen receptor6ERT2
HAhuman influenza virus HA (hemagglutinin) tag sequence6HA
Oxtroxytocin receptor6Oxtrendonuclease-mediated mutation 4, Yukiko U Inoue
T2AThosea asigna virus 2A peptide (insect)6T2A
icrephage P1 codon improved Cre (iCre)6icre
nlsSV40 large T antigen nuclear localization signal (NLS)6nls

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman influenza virus HA (hemagglutinin) tag sequence, Thosea asigna virus 2A peptide (insect), SV40 nls, Phage P1 Cre (codon-improved Cre recombinase), human Estrogen receptor (ERT2), mouse Oxtr genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. eNeuro, 9(1):ENEURO.0423-21.2022 (2022).For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorTakayoshi Inoue (National Center of Neurology and Psychiatry)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data