Strain Data Sheet

RBRC12152

Strain Information

Image
BRC No.RBRC12152
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Shh<tm1(cre)Itl>/Tshim
Former Common nameSHH-IRES-Cre (SHOG)
H-2 Haplotype
ES Cell lineiTL BA1[(C57BL/6N x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentTomomi Shimogori.Targeted iTL BA1 (C57BL/6 x 129/SvEv) hybrid embryonic stem cells were microinjected into C57BL/6 blastocysts. Resulting chimeras with a high percentage agouti coat color were mated to wild-type C57BL/6 mice to generate F1 heterozygous offspring.
Strain descriptionCre expression in SHH expressing cells
Colony maintenance
References
Dynamic spatiotemporal gene expression in embryonic mouse thalamus.
Asuka Suzuki-Hirano, Masaharu Ogawa, Ayane Kataoka, Aya C Yoshida, Daisuke Itoh, Masaki Ueno, Seth Blackshaw, Tomomi Shimogori
J. Comp. Neurol., 519(3):528-43 (2011). 21192082

Health Report

Examination Date / Room / Rack2025/11/04Room:3-3Rack:CSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site5Frt
Frt yeast FRT (flippase recombination target) site5Frt
Ires internal ribosomal entry site (EMCV)5
Shh
MGI:98297		sonic hedgehog5Shh<tm1(cre)Tshim>
MGI:5294343		targeted mutation 1, Tomomi Shimogori
  • holoprosencephaly 3(DisGeNET, MedGEN)

  • microphthalmia, isolated, with coloboma 5(MedGEN)

  • schizencephaly(MedGEN)
    • more 1 Diseases
  • solitary median maxillary central incisor syndrome(MedGEN)
    • cre Phage P1 Cre recombinase5cre
    neo neomycin resistance gene (E. coli)5 mouse Phosphoglycerate kinase (PGK) promoter

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAEncephalomyocarditis virus (Cardiovirus) IRES, P1 Phage Cre, Saccaromyces cerevisiae FRT sites, mouse Phosphoglycerate kinase (PGK) promoter, E. coli neomycin resistance gene, mouse Shh genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Cre driver
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Comp. Neurol., 519(3):528-43 (2011). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorTomomi Shimogori (RIKEN CBS)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    Strain AvailabilityCryopreserved embryos (within 1 month)
    Live mouse (3 to 6 months)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data