Strain Data Sheet

RBRC12109

Strain Information

Image
BRC No.RBRC12109
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6(Cg)-Vip<em1(tTA2)Miem>
Former Common nameVip-tTA
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Cell Type-Specific Genetic Manipulation and Impaired Circadian Rhythms in Vip tTA Knock-In Mice.
Yubo Peng, Yusuke Tsuno, Ayako Matsui, Yuichi Hiraoka, Kohichi Tanaka, Shin-Ichi Horike, Takiko Daikoku, Michihiro Mieda
Front. Physiol., 13:895633 (2022). 35592033

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Vip
MGI:98933		vasoactive intestinal polypeptide10Vip
tTA2 synthetic modified tetracycline-regulated transactivator: E. coli TetR (the repressor of tetracycline resistance operon), herpes simplex virus VP16 transcription avtivating domain (C-terminal portion)10tTA2

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNAE. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain, SV40 Poly A signal
Research applicationTet system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Front. Physiol., 13:895633 (2022).
DepositorMichihiro Mieda (Kanazawa University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data