Strain Data Sheet
RBRC11928
Strain Information | |
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| Image | |
| BRC No. | RBRC11928 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | C57BL/6N.FVB-Tg(Thy1-GCaMP6-actin/mCherry)1ShiSatm |
| Former Common name | B6N.FVB-Thy1-G6-actin-mCherry-Tg #6 |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | |
| Strain description | |
| Colony maintenance | |
| References | Genetically encoded green fluorescent Ca2+ indicators with improved detectability for neuronal Ca2+ signals. Ohkura M, Sasaki T, Sadakari J, Gengyo-Ando K, Kagawa-Nagamura Y, Kobayashi C, Ikegaya Y, Nakai J PLoS One. 7(12):e51286 (2012). 23240011 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| ACTB | Human beta actin | UN | ACTB | ||||
| F2A | foot-and-mouth disease virus (FMD) 2A peptide | UN | F2A | ||||
| GCaMP6 | RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA | UN | GCaMP6 | ||||
| WPRE | Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE) | UN | WPRE | ||||
| mCherry | Red Fluorescent Protein (Discosoma sp.) | UN | mCherry | ||||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | Mouse Thy1 promoter, G-CaMP6-actin(RSET tag, Chicken myosin light chain kinase (M13), Jellyfish circularly permutated EGFP (cpEGFP), Rat calmodulin (CaM), linker, Human beta actin), Discosoma sp. mCherry cDNA, Foot-and-Mouth Disease Virus 2A peptide (F2A), Mouse poly A, Woodchuck hepatitis virus WPRE |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One. 7(12):e51286. (2012).The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not—for profit institution for a not—for—profit research. The BIOLOGICAL RESOURCE must not be provided to any third party without DEPOSITOR's permission. |
| Depositor | Masaaki Sato (Hokkaido University) |
| Strain Status |  Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved sperm (2 to 4 months) Cryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |