Strain Data Sheet
RBRC11900
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11900 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6-Spic<tm1.2Ksho> |
| Former Common name | Spi-C KO |
| H-2 Haplotype | |
| ES Cell line | JM8A3.N1 [C57BL/6N-A<tm1Brd>] |
| Background strain | |
| Appearance | |
| Strain development | Developed by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005. The neomycin resistance gene was deleted and backrossed to C57BL/6 mice by Wataru Ise, Osaka University Immunology Frontier Research Center (IFReC), around 2011-2013. |
| Strain description | Spi-C is an Ets family transcription factor. In Spi-C KO mice, the Spi-C gene locus was disrupted by cre recombinase-mediated depletion of the exon-containing regions flanked by two loxP sites. Spi-C-deficient mice show various phenotypes, such as ablation of splenic red pulp macrophage and defects in intestinal macrophage functions. |
| Colony maintenance | |
| References | Heme ameliorates dextran sodium sulfate-induced colitis through providing intestinal macrophages with noninflammatory profiles. Hisako Kayama, Masako Kohyama, Daisuke Okuzaki, Daisuke Motooka, Soumik Barman, Ryu Okumura, Masato Muneta, Katsuaki Hoshino, Izumi Sasaki, Wataru Ise, Hiroshi Matsuno, Junichi Nishimura, Tomohiro Kurosaki, Shota Nakamura, Hisashi Arase, Tsuneyasu Kaisho, Kiyoshi Takeda Proc. Natl. Acad. Sci. USA, 115:8418-8423 (2018). 30061415 |
Health Report | |
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| Examination Date / Room / Rack | 2024/06/10Room:3-2Rack:D 2024/03/11Room:3-2Rack:D 2023/12/11Room:3-2Rack:D 2023/09/11Room:3-2Rack:D 2023/06/13Room:3-2Rack:D 2023/03/13Room:3-2Rack:D |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Spic | Spi-C transcription factor (Spi-1/PU.1 related) | 10 | Spic | targeted mutation 1.2, Tsuneyasu Kaisho | |||
| loxP | phage P1 loxP | 10 | loxP | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
|---|---|
| Donor DNA | Phage P1 loxP site, mouse Spic genomic DNA |
| Research application | Cre/loxP system |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA, 115:8418-8423 (2018).Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR (Dr. Tsuneyasu Kaisho) and CO-DEVELOPER (Dr. Wataru Ise) using the Approval Form (form D). (Contact info: Tsuneyasu Kaisho <tkaisho@wakayama-med.ac.jp>) The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research. For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. |
| Depositor | Tsuneyasu Kaisho (RIKEN) |
| Strain Status |  Live mice Frozen sperm |
| Strain Availability | Live mouse (3 to 6 months) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |