Strain Data Sheet

RBRC11845

Strain Information

Image
BRC No.RBRC11845
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Tg(HCRT-EGFP)E2Stak
Former Common nameOrexin-EGFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Hypothalamic orexin neurons regulate arousal according to energy balance in mice.
Akihiro Yamanaka, Carsten T Beuckmann, Jon T Willie, Junko Hara, Natsuko Tsujino, Michihiro Mieda, Makoto Tominaga, Ken ichi Yagami, Fumihiro Sugiyama, Katsutoshi Goto, Masashi Yanagisawa, Takeshi Sakurai
Neuron, 38(5):701-13 (2003). doi: 10.1016/s0896-6273(03)00331-3. 12797956

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman prepro-orexin promoter, jellyfish eGFP cDNA, mouse Prm1 polyA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Neuron. Jun 5;38(5):701-13 (2003). doi: 10.1016/s0896-6273(03)00331-3.
DepositorTakeshi Sakurai (University of Tsukuba)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data