Strain Data Sheet

RBRC11837

Strain Information

Image
BRC No.RBRC11837
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6;B6D2-Adra1a<em1Makot>
Former Common nameAdra1a-floxed mice
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
References
Efficient generation of conditional knockout mice via sequential introduction of lox sites.
Takuro Horii, Sumiyo Morita, Mika Kimura, Naomi Terawaki, Mihiro Shibutani, Izuho Hatada
Sci. Rep., 7(1):7891.(2017). 28801621

Spinal astrocytes in superficial laminae gate brainstem descending control of mechanosensory hypersensitivity.
Yuta Kohro, Tsuyoshi Matsuda, Kohei Yoshihara, Keita Kohno, Keisuke Koga, Ryuichi Katsuragi, Takaaki Oka, Ryoichi Tashima, Sho Muneta, Takuya Yamane, Shota Okada, Kazuya Momokino, Aogu Furusho, Kenji Hamase, Takumi Oti, Hirotaka Sakamoto, Kenichiro Hayashida, Ryosuke Kobayashi, Takuro Horii, Izuho Hatada, Hidetoshi Tozaki-Saitoh, Katsuhiko Mikoshiba, Verdon Taylor, Kazuhide Inoue, Makoto Tsuda
Nat. Neurosci., 23(11):1376-1387 (2020). 33020652

Health Report

Examination Date / Room / Rack2024/05/20Room:4-ARack:D

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Adra1aadrenergic receptor, alpha 1a14Adra1aendonuclease-mediated mutation 1, Makoto Tsuda
loxPphage P1 loxP14loxP
loxPphage P1 loxP14loxP

Phenotype

Annotation by Mammalian phenotyhpe ontology
Detailed phenotype data

Ordering Information

Donor DNAphage P1 loxP sites
Research applicationCre/loxP system
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 7(1):7891.(2017). Nat. Neurosci., 23(11):1376-1387 (2020).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In the event that the RECIPIENT or the RECIPIENT SCIENTIST intends to publish and/or present, in the form of papers or presentations at academic conferences or otherwise (the “Publications”), about the result of the Research using the MATERIAL, the RECIPIENT and the RECIPIENT SCIENTIST shall include in such Publications the acknowledgment: “Adrala-floxed mice were kindly provided by Dr. Izuho Hatada (Gunma University, Japan)".
DepositorMakoto Tsuda (Kyushu University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data