Strain Data Sheet

RBRC11734

Information1

Image
BRC No.RBRC11734
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6-Tg(Nodal NDE-5HT6/GCaMP6,RGECO1)130AHmd
Former Common nameNDE4-hsp-5HT6GCaMP6-2A-RGECO1(130A)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesSci. Adv., 6(30):eaba1195 (2020). doi: 10.1126/sciadv.aba1195. 32743070

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GCaMP6RSET tag, chicken myosin light chain kinase (M13) cDNA, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNAUNGCaMP6

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptideUNP2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
RGECO1red fluorescent protein (Discosoma sp.)UNRGECO1

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyAUN

Information2

Donor DNAオワンクラゲ 蛍光タンパク質遺伝子(GFP誘導体GCaMP6), 造礁サンゴ(イソギンチャクモドキ) DsRed 誘導体 RGECO1, マウス Nodal 遺伝子エンハンサー配列(NDE), 発現調節用プロモーター配列(Hsp68), セロトニンレセプター遺伝子 (5-HT6), porcine teschovirus-1 ポリペプチド自己切断配列(2A cleavage site), SV40 ポリA付加配列
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Adv., 6(30):eaba1195 (2020). doi: 10.1126/sciadv.aba1195.
DepositorHiroshi Hamada (RIKEN BDR)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data