Strain Data Sheet

RBRC11719

Information1

Image
BRC No.RBRC11719
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Vim<tm1Sibg>
Former Common nameVIM<SA/SA>
H-2 Haplotype
ES Cell lineEB3 [129/Ola]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesJ. Biol. Chem., 288(50):35626-35 (2013). 24142690

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Vimvimentin2Vim<tm1Sibg>targeted mutation 1, Shigeyoshi Itohara

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP2loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)2

Information2

Donor DNAphage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neomycin resistant gene, mouse Vim genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 288(50):35626-35 (2013).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Masaki Inagaki is requested.
DepositorDaishi Yamakawa (Mie University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data