Strain Data Sheet

RBRC11718

Information1

Image
BRC No.RBRC11718
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Tchp<tm1Inag>
Former Common nameTchp-/-
H-2 Haplotype
ES Cell lineEB3 [129/Ola]
Background strain
Appearance
Strain development
Strain description
Colony maintenance
ReferencesCell Rep., 34(10):108817 (2021). 33691104

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Tchptrichoplein, keratin filament binding5Tchp<tm1Inag>targeted mutation 1, Masaki Inagaki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
En2En2 SA (mouse En2 intron 2/exon 3 splice acceptor sequence)5En2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)5

Information2

Donor DNAmouse En2 genomic DNA, EMCV IRES, E. coli Beta galactosidase, E. coli Neomycin resistant gene, mouse Tchp genomic DNA
Research applicationCancer Research
Cell Biology Research
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Rep. Mar 9;34(10):108817 (2021)In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Masaki Inagaki is requested.
DepositorDaishi Yamakawa (Mie University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data