Strain Data Sheet

RBRC11677

Information1

Image
BRC No.RBRC11677
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Fosb<tm2.1Yun>
Former Common nameFosb<G>
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeposited by Yusaki Nakabeppu, Kyusyu University in 2022. This strain was generated using CCE/EK.CCE ES Cell derived from 129S/SvEv-Gpi1<c> and then mice were backcrossed to C57BL/6J for 41 generations. C57BL/6J congenic strain.
Strain descriptionA destabilized variant of the enhanced green fluorescent protein (EGFP) was inserted in frame in exon 2 and the remainder of exon 2 and all of exon 3 were replaced by a floxed neo cassette via homologous recombination. Cre mediated recombination removed the neo cassette.
Colony maintenance
ReferencesMol. Biol. Cell., 19(11):4717-29 (2008). 18753407
Neuropsychopharmacology, 38(5):895-906 (2013). 23303048

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FosbFBJ osteosarcoma oncogene B7Fosb<tm2.1Yun>targeted mutation 2.1, Yusaku Nakabeppu

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
d2EGFPEGFP destabilized by residues 422-461 of mouse ornithinedecarboxylase7d2EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Information2

Donor DNABacteriophage P1 loxP, Jellyfish/mouse d2EGFP (EGFP destabilized by residues 422-461 of mouse ornithinedecarboxylase)
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell., 19(11):4717-29 (2008).
DepositorYusaku Nakabeppu (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

BRC mice in Publications

No Data