Strain Data Sheet

RBRC11676

Information1

Image
BRC No.RBRC11676
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S-Fosb<tm1.1Yun>
Former Common nameFosb<d>
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeposited by Yusaki Nakabeppu, Kyusyu University in 2022. This strain was generated using CCE/EK.CCE ES Cell derived from 129S/SvEv-Gpi1<c> and then mice were backcrossed to C57BL/6J for 46 generations. C57BL/6J congenic strain.
Strain descriptionA floxed neo cassette was inserted in intron 3 and the sequence encoding the alternative splicing donor site for the exonic intron in exon 4 was replaced with tandem stop codons (GTG, AGA to TAG, TGA) via homologous recombination. This prevents the expression of the short transcript but allows expression of the longer transcript that encodes two isoforms (deltaFosb and delta2deltaFosb). Cre-mediated recombination removed the neo cassette.
Colony maintenance
ReferencesMol. Biol. Cell., 19(11):4717-29 (2008). 18753407
Biol. Psychiatry, 70(5):487-95 (2011). 21679928

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FosbFBJ osteosarcoma oncogene B7Fosb<tm1.1Yun>targeted mutation 1.1, Yusaku Nakabeppu

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Information2

Donor DNABacteriophage P1 loxP
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol. Biol. Cell., 19(11):4717-29 (2008).
DepositorYusaku Nakabeppu (Kyushu University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data