Strain Data Sheet
RBRC11665
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11665 |
| Type | CRISPR/Cas9 (Transgene) |
| Species | Mus musculus |
| Strain name | B6D2-Mtl5<em1Osb> Tg(Clgn-Tesmin/BioID2/3xFLAG)1Osb |
| Former Common name | Tesmin<-316/-316> BioID2 Tg |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from RBRC09987 were used to generate this line. Mixed genetic background. |
| Strain description | Compound strain harboring a transgene expressing C-terminally tagged Tesmin under the control of Clgn promoter (BioID2/3xFLAG tags). This strain also has mutation in the Kctd19 gene. 316 bp deletion in the Tesmin gene. |
| Colony maintenance | |
| References | Proximity-dependent biotin labeling in testicular germ cells identified TESMIN-associated proteins. Seiya Oura, Akinori Ninomiya, Fuminori Sugihara, Martin M Matzuk, Masahito Ikawa Sci. Rep., 12(1):22198 (2022). 36564444 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Tesmin | testis expressed metallothionein like | 19 | Tesmin | endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University | |||
| BioID2 | proximity-dependent biotin identification 2 | UN | BioID2 | ||||
| FLAG | FLAG tag (synthetic) | UN | FLAG | ||||
| Tesmin | testis expressed metallothionein like | UN | Tesmin | mouse Clgn promoter | |||
Phenotype | |
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| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | human hU6 promoter, CMV CMV enhancer (CBh), chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Mouse a part of Tesmin gene, mouse Clgn promoter, mouse Tesmin cDNA, synthetic Flag tag, Aquifex aeolicus Biotin ligase (BirA R40G), rabbit globin polyA signal |
| Research application | |
| Specific Term and Conditions | The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Sci. Rep., 12(1):22198 (2022).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again. |
| Depositor | Masahito Ikawa (Osaka University) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
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BRC mice in Publications |
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| No Data |