Strain Data Sheet



BRC No.RBRC11663
SpeciesMus musculus
Strain nameB6D2-Fam209<em1Osb>
Former Common nameFam209<-3/wt>
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionFAM209 mutant mouse generated by the CRISPR/Cas9. This strain lacks 43th amino acid of Fam209 protein. Homozygous males are infertile.
Colony maintenance
ReferencesJ. Cell Sci., 134(21):jcs.259206. doi: 10.1242/jcs.259206. (2021). 34471926

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Fam209family with sequence similarity 2092Fam209<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University


Donor DNAhuman hU6 promoter, CMV CMV enhancer, chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistant gene, Mouse a part of Fam209 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 134(21):jcs.259206. doi: 10.1242/jcs.259206. (2021).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data