Strain Data Sheet

RBRC11663

Strain Information

Image
BRC No.RBRC11663
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6D2-Fam209<em1Osb>
Former Common nameFam209<-3/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionFAM209 mutant mouse generated by the CRISPR/Cas9. This strain lacks 43th amino acid of Fam209 protein. Homozygous males are infertile.
Colony maintenance
References
FAM209 associates with DPY19L2, and is required for sperm acrosome biogenesis and fertility in mice.
Julio M Castaneda, Keisuke Shimada, Yuhkoh Satouh, Zhifeng Yu, Darius J Devlin, Masahito Ikawa, Martin M Matzuk
J. Cell Sci., 134(21):jcs259206 (2021). 34471926

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fam209
MGI:1923676		family with sequence similarity 2092Fam209<em1Osb> endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNAhuman hU6 promoter, CMV CMV enhancer, chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Mouse a part of Fam209 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Cell Sci., 134(21):jcs.259206. doi: 10.1242/jcs.259206. (2021).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data