Strain Data Sheet



BRC No.RBRC11657
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6D2-Spaca4<tm1Osb>
Former Common nameSpaca4<tm1Osb>
H-2 Haplotype
ES Cell lineEGR-G101 [C57BL/6NCr-Tg(CAG/Acr-EGFP)C3-N01-FJ002Osb]
Background strain
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. EGR-G101 ES cells derived from a Tg mouse in C57BL/6N background were used. Mice were crossed to B6D2F1. Mixed genetic background.
Strain descriptionSpaca4 knockout mice.
Colony maintenance
ReferencesProc. Natl. Acad. Sci. USA, 118(39):e2108777118 (2021). doi: 10.1073/pnas.2108777118. 34556579

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Spaca4sperm acrosome associated 47Spaca4<tm1Osb>targeted mutation 1, Research Institute for Microbial Diseases, Osaka University


Donor DNAMouse Spaca4 gDNA, E. coli Neomysin phosphotransferase gene (neoR), Mouse PGK promoter, Eschericia coli Ampicillin resistant gene, Phage P1LoxP site, Yeast FRT site, Herpes simplex virus Thymidine kinase (HSV-TK), Mouse PGK polyA site
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA, 118(39):e2108777118 (2021). doi: 10.1073/pnas.2108777118.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data