Strain Data Sheet

RBRC11647

Strain Information

Image
BRC No.RBRC11647
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6D2-Tg(Clgn-Fam71f1/PA)1Osb
Former Common nameClgn-Fam71f1-PA Tg
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from RBRC11477 were used to generate this line. Mixed genetic background.
Strain descriptionTransgenic mice expressing PA-fused Fam71f1 under the control of Clgn promoter.
Colony maintenance
References
FAM71F1 binds to RAB2A and RAB2B and is essential for acrosome formation and male fertility in mice.
Morohoshi A, Miyata H, Oyama Y, Oura S, Noda T, Ikawa M
Development, 148(21):dev199644 (2021). 34714330

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Fam71f1 family with sequence similarity 71, member F1UNFam71f1 mouse Clgn promoter
PA PA tag (GVAMPGAEDDVV) (synthetic) human PodoplaninUNPA

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNAMouse Calmegin promoter, Mouse Fam71f1 cDNA, Synthetic PA tag, Rabbit globin polyA signal, human hU6 promoter, CMV CMV enhancer, chicken chicken beta-actin promoter (CBh), SV40 SV40 nuclear localization signal, Streptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNA, Escherichia coli ampicillin resistance gene, Mouse a part of Fam71f1 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Developmen, 148(21):dev199644 (2021). doi: 10.1242/dev.199644. Epub 2021 Oct 29.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
Co-creation Bureau, Osaka University HP

BRC mice in Publications

No Data