Strain Data Sheet

RBRC11637

Information1

Image
BRC No.RBRC11637
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Kctd19<em1Osb> Tg(Clgn-3xFLAG-Kctd19)1Osb Tg(Clgn-Kctd19/3xHA)2Osb
Former Common nameKctd19<del/del>, Tg#2 (FLAG/HA)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from RBRC11037 were used to generate this line. Mixed genetic background.
Strain descriptionCompound strain harboring two transgenes expressing C-terminally tagged Kctd19 under the control of Clgn promoter (3xFLAG and 3xHA tags). This strain also has mutation in the Kctd19 gene. 9620 bp deletion in the Kctd19 gene.
Colony maintenance
ReferencesPLoS Genet., 17(5):e1009412 (2021). 33961623

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kctd19potassium channel tetramerisation domain containing 198Kctd19<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLAGFLAG tag (synthetic)UNFLAGmouse Clgn promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kctd19potassium channel tetramerisation domain containing 19UNKctd19mouse Clgn promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kctd19potassium channel tetramerisation domain containing 19UNKctd19

Information2

Donor DNAStreptococcus pyogenes　crRNA, tracrRNA, Mouse a part of Kctd19 gene, Mouse calmegin promoter, Rabbit beta-globin polyadenylation signal Mouse Kctd19 cDNA, Influenza virus Influenza hemagglutinin (HA) tag, synthetic FLAG tag
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 17(5):e1009412 (2021). doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data