Strain Data Sheet

RBRC11635

Strain Information

Image
BRC No.RBRC11635
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6D2-Abcc9<em2(Abcc9/Cre/ERT2)Osb>
Former Common nameAbcc9-CreERT2<KI/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain descriptionCre/ERT2 knock-in mice at C-termini of the Abcc9 via P2A. Line 2.
Colony maintenance
References
KCNJ8/ABCC9-containing K-ATP channel modulates brain vascular smooth muscle development and neurovascular coupling.
Koji Ando, Lei Tong, Di Peng, Elisa Vázquez-Liébanas, Hirohisa Chiyoda, Liqun He, Jianping Liu, Koichi Kawakami, Naoki Mochizuki, Shigetomo Fukuhara, Jaime Grutzendler, Christer Betsholtz
Dev. Cell., 57(11):1383-1399.e7 (2022). 35588738

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Abcc9
MGI:1352630		ATP-binding cassette, sub-family C member 96Abcc9<em2(Abcc9/Cre/ERT2)Osb> endonuclease-mediated mutation 2, Research Institute for Microbial Diseases, Osaka University
  • atrial fibrillation, familial, 12(MedGEN)

  • dilated cardiomyopathy 1O(MedGEN)

  • hypertrichotic osteochondrodysplasia Cantu type(DisGeNET, MedGEN)
    • ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)6ERT2
    cre Phage P1 Cre recombinase6cre

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAStreptococcus pyogenes crRNA, tracrRNA, Mouse a part of Abcc9 gene, Phage P1 Cre recombinase, Human ERT2, ER with the triple mutation G400V/M543A/L544A,Picornavirus P2A self-cleaving peptide coding sequence, Woodchuck hepatitis virus Woodchuck hepatitis virus Posttranscriptional Regulatory Element (WRPE), Bovine Bovine growth hormone polyadenylation seqence (BGH polyA), Escherichia coli Lac Z, T7 phage T7 promoter, T3 phage T3 promoter, Escherichia coli lac promoter,Escherichia coli ampicillin resistance gene
    Research applicationCre/loxP system
    Cre driver
    Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Dev. Cell., 57(11):1383-1399.e7 (2022).RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
    DepositorMasahito Ikawa (Osaka University)
    Strain Statusan icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Cre-ERT(2): NOTICE TO RECIPIENT
    5. Acceptance of responsibility for living modified organism (Japanese / English)
    Lab HP, Co-creation Bureau, Osaka University HP

    BRC mice in Publications

    No Data