Strain Data Sheet

RBRC11630

Information1

Image
BRC No.RBRC11630
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameSTOCK Zfp541<em1Osb>
Former Common nameZfp541<-26118/wt>
H-2 Haplotype
ES Cell lineEGR-G01 [129S2 x C57BL/6NCr-Tg(CAG/Acr-EGFP)]
Background strain
Appearance
Strain developmentDeposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2022. Mice were generated using EGR-G01 (ES cell) derived from (129S2 x B6Cr)F1-Tg(CAG/Acr-EGFP). Mice were further crossed to B6D2F1. Mixed genetic background.
Strain descriptionZfp541 KO mouse generated by the CRISPR/Cas9. 26,118 bp deletion in the IZfp541 gene. Homozygou males are infertile.
Colony maintenance
ReferencesReprod Med Biol., 22(1):e12507(2023). doi: 10.1002/rmb2.12507. 36845001
PLoS Genet., 17(5):e1009412 (2021). 33961623

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Zfp541zinc finger protein 5417Zfp541<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Information2

Donor DNAhuman hU6 promoterCMV CMV enhancer (CBh)chicken chicken beta-actin promoter (CBh)SV40 SV40 nuclear localization signalStreptococcus pyogenes SpCas9 (human codon-optimized), crRNA, tracrRNAEscherichia coli ampicillin resistant geneStreptomyces alboniger puromycin resistant geneMouse a part of Zfp541 gene
Research application
Specific Term and ConditionsThe RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS Genet., 17(5):e1009412 (2021). doi: 10.1371/journal.pgen.1009412. eCollection 2021 May.RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
DepositorMasahito Ikawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data