Strain Data Sheet

RBRC11578

Information1

Image
BRC No.RBRC11578
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.Cg-Hras<tm1.1(G12S)Tumg>
Former Common name
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Yoko Aoki and Shin-ichi Inoue, Tohoku University School of Medicine in 2021. BRUCE-4 ES cells were used. Mice were backcrossed to BALB/c for more than 10 generations.
Strain descriptionKnock-in mutants in which a point mutation (G12S) was introduced at Exon 1 of the Hras gene. Floxed cDNA/stop cassette and Frt-flnaked Neo cassette were removed by crossing with Cre mice. BALB/c congenic strain. C57BL/6 congenic strain is also available as RBRC10866.
Colony maintenance
ReferencesEBioMedicine, 27:138-150 (2018). 29254681

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HrasHarvey rat sarcoma virus oncogene7Hras<tm1.1(G12S)Tumg>targeted mutation 1.1, Yoko Aoki

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Information2

Donor DNABacteriophage P1 loxP site, mouse Hras genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. EBioMedicine. 27:138-150.(2018) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR. RECIPIENT must contact the DEPOSITOR in case of application for any patents or commercial use base on the results from the use of the BIOLOGICAL RESOURCE.
DepositorYoko Aoki (Tohoku University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data