Strain Information | |
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Image | |
BRC No. | RBRC11575 |
Type | Targeted Mutation |
Species | Mus musculus |
Strain name | C57BL/6N-Gt(ROSA)26Sor<tm1(CAG-G-CaMP9a,-R-CaMP2)Hbto> |
Former Common name | G-CaMP9a/R-CaMP2 KI mice |
H-2 Haplotype | |
ES Cell line | RENKA [C57BL/6NCrlCrlj] |
Background strain | |
Appearance | |
Strain development | Deposited by Haruhiko Bito and Masayuki Sakamoto, The Universtiy of Tokyo in 2022. This strain was generated using RENKA (ES cell) derived from C57BL/6NCrlCrlj. C57BL/6N genetic background. |
Strain description | G-CaMP9a and R-CaMP2 reporter mice under the control of Flp/FRT and Cre/loxP recombination, respectively.R-CaMP2 expression can be regulated by tTA/rtTA, but its expression is relatively weak. |
Colony maintenance | |
References | Cell Rep., Methods 2, 100168 (2022). 35474964 STAR Protoc., 3(2):101421 (2022). 35693213 |
Health Report | |
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Examination Date / Room / Rack | 2024/02/26Room:4-CRack:B 2023/11/27Room:4-CRack:B 2023/08/28Room:4-CRack:B 2023/05/29Room:4-CRack:B 2023/02/27Room:4-CRack:B 2022/11/29Room:4-CRack:B |
Gene | |
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Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Gt(ROSA)26Sorgene trap ROSA 26, Philippe Soriano6Gt(ROSA)26Sor<tm1(CAG-G-CaMP9a,-R-CaMP2)Hbto>targeted mutation 1, Haruhiko Bito Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Frtyeast FRT (flippase recombination target) site6Frt Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GCaMP9aRSET tag, ckkap, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA6GCaMP9aCAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter GHGrowth hormone polyA (human)6GH Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter His3His3 (S. cerevisiae)6His3 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter RCaMP2His-tag, T7-tag, Xpress-tag, ckkap, cpApple(circularly permuted red fluorescent protein (mApple)) cDNA, rat calmodulin (CaM) cDNA6RCaMP2 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter TRE3GTet-On 3G (transactivator gene)6TRE3G Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter WPREWoodchuck hepatitis virus posy-transcriptional regulatory element (WPRE)6WPRE Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter loxPphage P1 loxP6loxP Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter neoneomycin resistance gene (E. coli)6mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site6rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter roxBacteriophage D6 rox site6rox Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter SV40 polyA6 |
Ordering Information | |
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Donor DNA | attB1, CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Phage P1 loxP sites,yeast FRT (flippase recombination target) sites, 2 x pA, Stop (SV40 polyA), G-CaMP9a(RSET tag, ckkap, Jellyfish circularly permutated EGFP (cpEGFP) cDNA, rat calmodulin (CaM) cDNA), Woodchuck hepatitis virus posy-transcriptional regulatory element (WPRE), rabbit beta globin polyA signal, E.coli TRE3G(Tet-On 3G, transactivator gene), R-CaMP2 (His-tag, T7-tag, Xpress-tag, Ca2+/CaM-binding peptide derived from rat CaMKKα Val438–Phe463 (ckkap), Discosoma sp. cpApple(circularly permuted red fluorescent protein (mApple)) cDNA, rat calmodulin (CaM) cDNA), human GH polyA, Phage D6 rox sites, mouse Phosphoglycerate kinase promoter (PGK promoter), phage T7 gb2 promoter, E. coli neomycin resistance gene, synthetic polyA, attB2, yeast His3, mouse Gt(ROSA)26Sor genomic DNA |
Research application | Cre/loxP system FLP/frt system Fluorescent Proteins/lacZ System Tet system |
Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (will be announced.) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
Depositor | Haruhiko Bito (The University of Tokyo) |
Strain Status | Live mice Frozen embryos Frozen sperm |
Strain Availability | Recovery and QC required prior to distribution Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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