Strain Data Sheet

RBRC11555

Information1

Image
BRC No.RBRC11555
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameC57BL/6-Hcrt<tm1.1(FLP)Ksak>
Former Common nameOrexin-Flippase
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDepsoted by Akihiro Yamanaka, Nagoya University in 2021. This strain was generated RENKA ES cells derived from C57BL/6N.
Strain descriptionEGFP/2A/FLP knockin mice at the translation initiation codon of the Hcrt gene.
Colony maintenanceHeterozygote x Wild-type [or crossing to C57BL/6JJcl]
ReferencesElife, 8:e44927 (2019). 31159922

Health Report

Examination Date / Room / Rack2022/08/29Room:4-CRack:F

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Hcrthypocretin11Hcrt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)11EGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
FLPeyeast flippase recombinase11FLPe

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHgrowth hormone polyA (human)11GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)11T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nlsSV40 large T antigen nuclear localization signal (NLS)11nls

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
roxD6 Bacteriophage rox site11rox

Information2

Donor DNAAequorea victoria EGFP cDNA, Thosea asigna virus 2A peptide (insect), SV40 nls, Saccharomyces cerevisiae Flpe recombinase, Bacteriophage D6 rox site, human GH polyA signal, mouse Hcrt genomic DNA
Research applicationFLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, Recipient shall inform the DEPOSITOR in advance, and shall discuss and agree on the handing thereof. The RECIPIENT shall contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of BIOLOGICAL RESOURCE, and shall obtain a prior written consent from the DEPOSITOR.
DepositorAkihiro Yamanaka (Nagoya University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovery and QC required prior to distribution
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)
  5. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data