Strain Data Sheet
RBRC11546
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11546 |
| Type | Targeted Mutation |
| Species | Mus musculus |
| Strain name | B6;129S-Ppme1<tm1Crv> |
| Former Common name | Ppme1 +/- mouse |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Benjamin F Cravatt, The Scripps Research Institute in 2021. This strain was generated ES cells derived from 129S6/SvEv and crossed to C57BL/6. Mixed genetic background. |
| Strain description | Ppme1 knock out mice. Exon 7 was replaced with a neo cassette. Homozygous mutants are neonatally lethal. |
| Colony maintenance | |
| References | Targeted disruption of the PME-1 gene causes loss of demethylated PP2A and perinatal lethality in mice. Ortega-Gutierrez S, Leung D, Ficarro S, Peters E C, Cravatt B F PLoS One, 3(7):e2486 (2008). 18596935 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Ppme1 MGI:1919840 | protein phosphatase methylesterase 1 | 7 | Ppme1<tm1Crv> MGI:3804708 | targeted mutation 1, Benjamin J Cravatt | |||
| neo | neomycin resistance gene (E. coli) | 7 | |||||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | E. coli Neomycin resistnat gene, Mouse Ppme1 gene cDNA |
| Research application | |
| Specific Term and Conditions | In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. |
| Depositor | Benjamin F. Cravatt (The Scripps Research Institute) |
| Strain Status |  Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering: |
BRC mice in Publications |
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| No Data |