Strain Data Sheet

RBRC11539

Strain Information

Image
BRC No.RBRC11539
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6-Awat2<em1Akih>
Former Common nameAwat2 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Akio Khara in 2021. Mice were generated using C57BL/6J with the CRISPR/Cas9. C57BL/6J genetic background.
Strain descriptionAwat2 mutant mice. 12 bp insertion at exon 5 of the Awat2 gene.
Colony maintenance
ReferencesiScience, 24, 102478 (2021). 34113821
iScience, 24, 103160 (2021). 34622178

Health Report

Examination Date / Room / Rack2024/05/20Room:4-ARack:J
2024/02/19Room:4-ARack:J
2023/11/20Room:4-ARack:J
2023/08/21Room:4-ARack:J
2023/05/22Room:4-ARack:J
2023/02/20Room:4-ARack:J
2022/11/21Room:4-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Awat2acyl-CoA wax alcohol acyltransferase 2XAwat2<em1Akih>endonuclease-mediated mutation 1, Akio Kihara

Ordering Information

Donor DNAS. pyogenes Cas9* * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan).
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkio Kihara (Hokkaido University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data