Strain Data Sheet


Strain Information

BRC No.RBRC11538
SpeciesMus musculus
Strain nameB6-Awat1<em1Akih>
Former Common nameAwat1 KO
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeposited by Akio Khara in 2021. Mice were generated using C57BL/6J with the CRISPR/Cas9. C57BL/6J genetic background.
Strain descriptionAwat1 mutant mice. 11 bp insertion at exon 3 of the Awat1 gene.
Colony maintenance
ReferencesiScience, 24, 103160 (2021). 34622178
iScience, 24, 102478 (2021). 34113821

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Awat1acyl-CoA wax alcohol acyltransferase 1XAwat1<em1Akih>endonuclease-mediated mutation 1, Akio Kihara

Ordering Information

Donor DNAS. pyogenes Cas9* * Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan).
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkio Kihara (Hokkaido University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (more than 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data