Strain Data Sheet
RBRC11535
Information1 | |
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| Image | |
| BRC No. | RBRC11535 |
| Type | Transgene |
| Species | Mus musculus |
| Strain name | B6;129B6-Tg(CAG-Su9/tdTomato/T2A/Puro)1GAitn |
| Former Common name | Y-mtRFP |
| H-2 Haplotype | |
| ES Cell line | |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Ayako Isotani, Nara Institute of Science and Technology in 2021. This strain was generated using mF1-05 ES cells derived from (129X1 x C57BL/6J)F1. Mixed genetic background. |
| Strain description | Reporter mice indicating the presence of Y chromosome. CAG-Su9-tdTomato-T2A-Puror is integrated at the Ddx3y locus. |
| Colony maintenance | |
| References | Exp Anim. Sep 20. (2021) 34544911 |
Health Report | |
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| Examination Date / Room / Rack | |
Gene | |
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| Gene info | Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Su9mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]YSu9CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA) Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter T2AThosea asigna virus 2A peptide (insect)YT2A Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter puropuromycin acyltransferase (Streptomyces alboniger)Ypuro Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter tdTomatotandem dimeric Red Fluorescent Protein (Discosoma sp.)YtdTomato |
Information2 | |
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| Donor DNA | CAG promoter (Cytomegalovirus CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Discosoma sp. Red Fluorecent Protein cDNA (tdTomato), Mouse Atp5g1 gene mitochondria localization signal(su9), Thosea asigna ribosomal skip site (T2A), Streptomyces alboniger Puromycin resistant gene |
| Research application | Fluorescent Proteins/lacZ System |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp Anim. Sep 20. (2021) doi: 10.1538/expanim.21-0119.For use of the BIOLOGICAL RESOURCE by a for-profit institution and an academic institution for the purpose of profits, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. |
| Depositor | Ayako Isotani (Nara Institute of Science and Technology) |
| Strain Status |  Frozen embryos |
| Strain Availability |  Recovery and QC required prior to distribution |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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