Strain Data Sheet

RBRC11535

Strain Information

Image
BRC No.RBRC11535
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;129B6-Tg(CAG-Su9/tdTomato/T2A/Puro)1GAitn
Former Common nameY-mtRFP
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Ayako Isotani, Nara Institute of Science and Technology in 2021. This strain was generated using mF1-05 ES cells derived from (129X1 x C57BL/6J)F1. Mixed genetic background.
Strain descriptionReporter mice indicating the presence of Y chromosome. CAG-Su9-tdTomato-T2A-Puror is integrated at the Ddx3y locus.
Colony maintenance
ReferencesExp Anim. Sep 20. (2021) 34544911

Health Report

Examination Date / Room / Rack2024/04/08Room:3-BRack:B
2024/01/09Room:3-BRack:B
2023/10/10Room:3-BRack:B

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Su9mitochondria localization signal of mouse Atp5g1[ATP synthase Fo complex subunit 9 (su9)]YSu9CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)YT2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
puropuromycin acyltransferase (Streptomyces alboniger)Ypuro

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
tdTomatotandem dimeric Red Fluorescent Protein (Discosoma sp.)YtdTomato

Ordering Information

Donor DNACAG promoter (Cytomegalovirus CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Discosoma sp. Red Fluorecent Protein cDNA (tdTomato), Mouse Atp5g1 gene mitochondria localization signal(su9), Thosea asigna ribosomal skip site (T2A), Streptomyces alboniger Puromycin resistant gene
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Exp Anim. Sep 20. (2021) doi: 10.1538/expanim.21-0119.For use of the BIOLOGICAL RESOURCE by a for-profit institution and an academic institution for the purpose of profits, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAyako Isotani (Nara Institute of Science and Technology)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: FP license of TBUSA
  3. CAGGS MTA (English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-
Mouse of the Month Jul 2023

BRC mice in Publications

No Data