Strain Data Sheet

RBRC11522

Information1

Image
BRC No.RBRC11522
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameB6-Cd19<tm1.1(icre)Yoba>
Former Common nameCD19-iCre
H-2 Haplotype
ES Cell lineEGR-101 [C57BL/6N]
Background strain
Appearance
Strain developmentDeposited by Yoshihiro Baba, Kyushu University in 2021. C57BL/6 genetic background.
Strain descriptioniCre knockin mice at the Cd19 locus. T2A-iCre was inserted at stop codon. FRT-flanked neo gne was deleted.
Colony maintenance
ReferencesSci. Rep., 11(1):5524 (2021) doi: 10.1038/s41598-021-84786-6. 33750849

Health Report

Examination Date / Room / Rack2022/07/11Room:3-ARack:J
2022/04/11Room:3-ARack:J
2022/01/24Room:3-ARack:J

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cd19CD19 antigen7Cd19<tm1.1(icre)Yoba>

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)7T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
icrephage P1 codon improved Cre (iCre)7icre

Information2

Donor DNAThosea asigna virus T2A, P1 phage iCre, Saccharomyces cerevisiae Frt site, mouse Cd19 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Yoshihiro Baba) and enter into a Material Transfer Agreement with Kyushu University. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorYoshihiro Baba (Kyushu University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data