Strain Data Sheet

RBRC11516

Information1

Image
BRC No.RBRC11516
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6J-Iars<em2(V79L)Nsas>
Former Common nameB6J-Iars V79L
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Nobuya Sasaki, Kitasato University in 2021. C57BL/6J genetic background.
Strain descriptionIars mutant mice harboring a point mutaion (V79L).
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iarsisoleucine-tRN A synthetase13Iars<em2(V79L)Nsas>endonuclease-mediated mutation 2, Nobuya Sasaki

Information2

Donor DNAmouse Iars1 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. Depending on the contact of the research, the DEPOSITOR may request collaboration.
DepositorNobuya Sasaki (Kitasato University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data