Strain Data Sheet

RBRC11514

Information1

Image
BRC No.RBRC11514
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6NJcl.Cg-Clstn3<tm1Tymo>
Former Common nameAlcadeinβ KO; Calsyntenin-3 KO
H-2 Haplotype
ES Cell lineD3 [129S2/SvPas]
Background strain
Appearance
Strain developmentDeposited by Toru Yamamoto, Kagawa University in 2021. This strain was generated using D3 ES cells derived from 129S2/SvPas. Mice were further backcrossed to C57BL/6N for 10 generations. C57BL/6N congenic strain.
Strain descriptionClstn3 knockout mice. PGK-neo fragment replaced exon 1 to 3 of the Clstn3 gene.
Colony maintenance
ReferencesJ. Biol. Chem., 295(28):9650-9662 (2020). doi: 10.1074/jbc.RA119.012386. 32467230
EMBO J., 26(6):1475-86 (2007). 17332754
J. Biol. Chem., 278(49):49448-58 (2003). 12972431
Traffic., 13(6):834-48 (2012). 22404616

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Clstn3calsyntenin 36Clstn3<tm1Tymo>targeted mutation 1, Toru Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)6mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAmouse phosphoglycerate kinase promoter (PGK promoter), E.coli Neo, PGK 3’end, mouse Clstn3 genomic DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J Biol Chem. 2020 Jul 10;295(28):9650-9662. doi: 10.1074/jbc.RA119.012386.
DepositorTohru Yamamoto (Kagawa University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data