Strain Data Sheet

RBRC11513

Information1

Image
BRC No.RBRC11513
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6NJcl.Cg-Clstn1<tm1.1Tymo>
Former Common nameAlcadeinα KO; Calsyntenin-1 KO
H-2 Haplotype
ES Cell lineTT2 [(C57BL/6NCrlj x CBA/JNCrlj)F1]
Background strain
Appearance
Strain developmentDeposited by Toru Yamamoto, Kagawa University in 2021. This strain was generated using TT2 ES cells derived from (C57BL/6 x CBA)F1. Mice were further backcrossed to C57BL/6N for 10 generations. C57BL/6N congenic strain. Frt-flanked Neo cassette was removed in this strain.
Strain descriptionClstn1 knockout mice with LacZ reporter replacing Exon 1 of the Clstn1 gene.
Colony maintenance
ReferencesJ. Biol. Chem., 295(28):9650-9662 (2020). doi: 10.1074/jbc.RA119.012386. 32467230
EMBO J., 26(6):1475-86 (2007). 17332754
J. Biol. Chem., 278(49):49448-58 (2003). 12972431
Traffic., 13(6):834-48 (2012). 22404616

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Clstn1calsyntenin 14Clstn1<tm1.1Tymo>targeted mutation 1.1, Toru Yamamoto

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBB2rabbit beta-globin 3’ end4HBB2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
frtyeast FRT (flippase recombination target) site4frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lacZbeta-galactosidase (E. coli)4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
lox71mutant lox sequence4lox71

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP4loxP

Information2

Donor DNAphage P1 lox71 site, E.coli LacZ, mouse PGK 3' end, Yeast frt site, rabbit beta-globin 3’end, phage P1 loxP site, mouse Clstn1 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. J. Biol. Chem., 295(28):9650-9662 (2020). doi: 10.1074/jbc.RA119.012386.In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the Ace.No. CDB0472K and Web. Site (http ://www2.clst.riken.jp/arg/mutant%20mice%20list.html) is requested. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the literature designed by the DEPOSITOR is requested (J. Biol. Chem., 295 9650-9662. doi:10.1074/jbc.RA119.012386). Please inform the DEPOSITOR (mutant.bdr@riken.jp) about the paper when published.
DepositorTohru Yamamoto (Kagawa University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data