Strain Data Sheet

RBRC11512

Information1

Image
BRC No.RBRC11512
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129P2-Utf1<tm1Okud>
Former Common nameUTF1 knockout mouse
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentStrain development: The targeting vector harboring neomycin resistance gene with SV40 poly-A addition signal under control of the endogenous UTF1 enhance-promoter was transferred to E14 ES cells to replace the 1st exon of UTF1 gene. The resultant UTF1 locus can produce no UTF1 protein (even the truncated form). Developer: Dr. Yo-ichi Nabeshima (National Institute of Neuroscience, Kodaira, Tokyo (1997), Present Affiliation :Foundation for Biomedical Research and Innovation at Kobe, Hyogo)Year of Development: 1997Year of Distribution: 1998
Strain descriptionThe prominent phenotype of UTF1 KO homozygous mice is “in utero growth retardation”. This phenotype is due to the growth retardation of placenta, not due to the defect of the differentiation program of embryo per se. This placental growth defect is unexpected because UTF1 gene is identified as a gene whose expression is pluripotent cell specific (Okuda, A. et al. (1998) EMBO J. 17: 2019–2032). This UTF1 KO phenotype indicates that this mouse strain may be useful to analyze not only the differentiation program of the embryo but also the placental developmental program.Homozygous mutant of C57BL/6J background is embryonic or peri-natal lethal. Some of the homozygous mutant mice of F1 (B6 and ICR) genetic background are viable. This phenotypic difference suggests that the severity of phenotype may be dependent on the genetic background (see Nishimoto,M. et al. (2013) PLoS ONE 8(7): e68119). Appearance: Black [a/a B/B C/C]Colony maintenance: Heterozygote crossing to C57BL/6JJcl
Colony maintenanceBackcross C57BL/6J (Jcl) : backcross to male C57BL/6J at least three generations. Homozygous mouse shows the “in utero growth retardation” phenotype.
ReferencesEMBO J. 17: 2019-2032 (1998). 9524124
PLoS One, 8(7):e68119 (2013). doi: 10.1371/journal.pone.0068119 23874519

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Utf1undifferentiated embryonic cell transcription factor 17Utf1<tm1Okud>targeted mutation 1, Akihiko Okuda

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA addition signal7

Information2

Donor DNAE. coli Neomycin resistance gene, SV40 PolyA addition signal, mouse Utf1 genomic DNA
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. PLoS One, 8(7):e68119 (2013). doi: 10.1371/journal.pone.0068119In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorAkihiko Okuda (Saitama Medical University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data