Strain Data Sheet

RBRC11476

update : 2024-04-19

Strain Information
Image
BRC No.	RBRC11476
Type	CRISPR/Cas9 (Transgene)
Species	Mus musculus
Strain name	B6D2-Fer1l5<em1(Fer1l5/PA/1D4)Osb>
Former Common name	Fer1l5<PA-1D4/wt>
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain development	Deposited by Masahito Ikawa, Research Institute for Microbial Diseases, Osaka University in 2021. Fertilized eggs derived from BDF1 × BDF1 were used to generate this line. Mixed genetic background.
Strain description	Knockin mice with PA-1D4-tag at C-terminus of the Fer1l5 gene.
Colony maintenance
References

Health Report
Examination Date / Room / Rack

Gene
Gene info	Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Fer1l5fer-1-like 5 (C. elegans)1Fer1l5<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter 1D41D4 tag (synthetic)11D4 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter Fer1l5fer-1-like 5 (C. elegans)1Fer1l5 Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter PAPA tag (GVAMPGAEDDVV) (synthetic) human Podoplanin1PA

Gene info

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Fer1l5fer-1-like 5 (C. elegans)1Fer1l5<em1Osb>endonuclease-mediated mutation 1, Research Institute for Microbial Diseases, Osaka University

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

1D41D4 tag (synthetic)11D4

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

Fer1l5fer-1-like 5 (C. elegans)1Fer1l5

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter

PAPA tag (GVAMPGAEDDVV) (synthetic) human Podoplanin1PA

Ordering Information
Donor DNA	Streptococcus pyogenes crRNA, tracrRNA, Mouse a part of Fer1l5gene, synthetic PA tag, synthetic 1D4 tag, Escherichia coli replication origin, Escherichia coli ampicillin resistant gene, Escherichia coli ampicillin resistant promoter gene, Escherichia coli lac promoter, Escherichia coli Lac operator, chicken beta-actin promoter, rabbit beta-globin polyadenylation signal, CMV CMV IE enhancer
Research application
Specific Term and Conditions	The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. 引用論文なしRECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University. The RECIPIENT which wants to use the BIOLOGICAL RESOURCE even after five years must obtain a written consent from the DEPOSITOR again.
Depositor	Masahito Ikawa (Osaka University)
Strain Status	Frozen sperm
Strain Availability	Recovery and QC required prior to distribution
Additional Info.	Necessary documents for ordering: Approval form (Japanese / English) Order form (Japanese / English) Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English) Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data