Strain Data Sheet

RBRC11440

Information1

Image
BRC No.RBRC11440
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameJF1.129P2(B6)-Kdm6b<tm1.1Masn>
Former Common name
H-2 Haplotype
ES Cell lineE14.1 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Masahide Asano, Kyoto University in 2021. Mice were generated using the E14 ES cells derived from 129P2/OlaHsd and the backcrossed to Jf1 for 7 generations.
Strain descriptionJmjd3(Kdm6b)knockout mice in JF1 congenic background. Exon 18 was deleted.
Colony maintenance
ReferencesGenome Res., 23(8), 1329-1338 (2013). 23604024
FASEB J., 31(6):2252-2266 (2017). doi: 10.1096/fj.201600642R. 28188179

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Kdm6bKDM1 lysine (K)-specific demethylase 6B11Kdm6b<tm1.1Masn>targeted mutation 1.1, Masahide Asano

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site11Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)11

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP11loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)11

Information2

Donor DNABacteriophage P1 loxP sites, Saccharomyces cerevisiae FRT sites, Mouse En-2 splicing acceptor, encephalomyocarditis virus Internal ribosomal entry site, E. coli Neomycin resistant gene, Bovine polyA signal, mouse Kdm6b genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J. 2017 Jun;31(6):2252-2266. doi: 10.1096/fj.201600642R. Genome Res. 2013 Aug;23(8):1329-38.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact Kyoto University and National Institute of Genetics in the case of application for an patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to Dr. Masahide Asano, Dr. Chie Naruse and Dr. Tsuyoshi Koide is requested. (Contact info: Dr. Masahide Asano <asano@anim.med.kyoto-u.ac.jp>, Institute of Laboratory Animals Graduate School of Medicine Kyoto University and Dr. Tsuyoshi Koide Ph.D. <tkoide@nig.ac.jp> Mouse Genomics Resource Laboratory, National Institute of Genetics)
DepositorMasahide Asano (Kyoto University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data