Strain Data Sheet

RBRC11439

Information1

Image
BRC No.RBRC11439
TypeTransgeneCartagena
SpeciesMus musculus
Strain nameB6;B6D2-Tg(CAG-OsTIR1-P2A-mAID-EGFP-nanoluc)#19Ysa
Former Common namepMK411-#19
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Yumiko Saga, National Institute of Genetics in 2021. This strain was generated using fertilized eggs derived from BDF1, then backcrossed to C57BL/6J twice.
Strain descriptionTransgenic mice (Line 19) expressing EGFP reporter and nanoluc fused with OsTIR and AID.
Colony maintenance
ReferencesNat. Commun., 11(1):5701 (2020). doi: 10.1038/s41467-020-19532-z. 33177522

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
P2Aporcine teschovirus-1 2A peptideUNP2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
TIR1F-box transport inhibitor response 1 (Oryza sativa)UNTIR1CAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA)

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
mAIDmAID (Arabidopsis thaliana)UNmAID

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
nanolucluciferase (Oplophorus gracilirostris)UNnanoluc

Information2

Donor DNACAG promoter (CMV-IE enhancer, chicken beta-actin promoter, rabbit beta-globin genomic DNA), Oryza sativa TIRI(F74G) cDNA, Porcie teschovirus P2A, Arabidopsis thaliana mAID cDNA, Aequorea victoria EGFP cDNA, Oplophorus gracilirostris luciferase cDNA, SV40 pA
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Nat. Commun., 11(1):5701 (2020). doi: 10.1038/s41467-020-19532-z.The RECIPIENT agree to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
DepositorYumiko Saga (National Institute of Genetics)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. CAGGS MTA (English)
  5. Acceptance of responsibility for living modified organism (Japanese / English)
  6. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data