Strain Data Sheet

RBRC11437

Information1

Image
BRC No.RBRC11437
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Islr<tm1.1(ZsGreen/DTR/cre)Mat>
Former Common nameMeflin-ZDC
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeposited by Atsushi Enomoto and Masahide Takahashi, Nagoya University in 2021. This strain was generated using CSL3 (ES cell) derived from 129S6/SvEvTac and then backcrossed to C57BL/6J for 6 generations.
Strain descriptionKnockin mice harboring ZsGreen-t2a-Diphtheria toxin receptor (DTR)-t2a-Cre by replacing Islr open reading frame.
Colony maintenance
ReferencesCancer Res., 79(20):5367-5381 (2019).doi: 10.1158/0008-5472.CAN-19-0454. 31439548
Circ Res. Aug 2;125(4):414-430.(2019)　doi: 10.1161/CIRCRESAHA.119.314806. 31221024

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Islrimmunoglobulin superfamily containing leucine-rich repeat9Islr<tm1.1(ZsGreen/DTR/cre)Mat>targeted mutation 1.1,Masahide TakahashiMeflin

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBEGFheparin-binding EGF-like growth factor (human)9HBEGF

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
T2AThosea asigna virus 2A peptide (insect)9T2A

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ZsGreenGreen Fluorescent Protein (Zoanthus sp.)9ZsGreen

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase9cre

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 PolyA signal9

Information2

Donor DNAZoanthus sp ZsGreen cDNA, Human DTR cDNA, Thosea asigna virus T2A, P1 bacteriophage Cre, Simian virus 40 PolyA signal, Saccharomyces cerevisiae FRT site, mouse Islr genomic DNA
Research applicationCre/loxP system
Fluorescent Proteins/lacZ System
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Circ Res. 2019 Aug 2;125(4):414-430. doi: 10.1161/CIRCRESAHA.119.314806.
Cancer Res. 2019 Oct 15;79(20):5367-5381. doi: 10.1158/0008-5472.CAN-19-0454.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT shall reach an agreement with the DEPOSITOR on terms and conditions of use of the BIOLOGICAL RESOURCE and shall obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for an patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAtsushi Enomoto (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: FP license of TBUSA
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data