Strain Data Sheet

RBRC11436

Strain Information

Image
BRC No.RBRC11436
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Islr<tm1.1(cre/ERT2)Mat>
Former Common nameMeflin-CreERT2
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Appearance
Strain developmentDeposited by Atsushi Enomoto and Masahide Takahashi, Nagoya University in 2021. This strain was generated using CSL3 (ES cell) derived from 129S6/SvEvTac and mice were backcrossed to C57BL/6J for more than 8 generations.
Strain descriptionCre/ERT2 konckin mice in which an open reading frame of the Islr gene was replaced with Cre/ERT2.
Colony maintenance
References
Meflin-Positive Cancer-Associated Fibroblasts Inhibit Pancreatic Carcinogenesis.
Yasuyuki Mizutani, Hiroki Kobayashi, Tadashi Iida, Naoya Asai, Atsushi Masamune, Akitoshi Hara, Nobutoshi Esaki, Kaori Ushida, Shinji Mii, Yukihiro Shiraki, Kenju Ando, Liang Weng, Seiichiro Ishihara, Suzanne M Ponik, Matthew W Conklin, Hisashi Haga, Arata Nagasaka, Takaki Miyata, Makoto Matsuyama, Tomoe Kobayashi, Tsutomu Fujii, Suguru Yamada, Junpei Yamaguchi, Tongtong Wang, Susan L Woods, Daniel Worthley, Teppei Shimamura, Mitsuhiro Fujishiro, Yoshiki Hirooka, Atsushi Enomoto, Masahide Takahashi
Cancer Res., 79(20):5367-5381 (2019). 31439548

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
ERT2 estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)9ERT2
Frt yeast FRT (flippase recombination target) site9Frt
HBB human beta-globin polyA9HBB
Islr
MGI:1349645		immunoglobulin superfamily containing leucine-rich repeat9Islr<tm1.1(cre/ERT2)Mat> targeted mutation 1.1,Masahide Takahashi
cre Phage P1 Cre recombinase9cre

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
  • increased bone ossification (MP:0020039)

  • postnatal growth retardation (MP:0001732)
    • Detailed phenotype data

    Ordering Information

    Donor DNAP1 bacteriophage Cre, Human ERT2 cDNA, Human beta-globin polyA, Saccharomyces cerevisiae FRT site, Mouse Islr (knock-in)genomic DNA
    Research applicationCre/loxP system
    FLP/frt system
    Cre driver
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Res. 2019 Oct 15;79(20):5367-5381. doi: 10.1158/0008-5472.CAN-19-0454.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT shall reach an agreement with the DEPOSITOR on terms and conditions of use of the BIOLOGICAL RESOURCE and shall obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for an patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
    DepositorAtsushi Enomoto (Nagoya University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    4. Cre-ERT(2): NOTICE TO RECIPIENT
    5. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-
    Mouse of the Month Jun 2022

    BRC mice in Publications

    No Data