Strain Data Sheet



BRC No.RBRC11436
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6.129S6-Islr<tm1.1(cre/ERT2)Mat>
Former Common nameMeflin-CreERT2
H-2 Haplotype
ES Cell lineCSL3[129S6/SvEvTac]
Background strain
Strain developmentDeposited by Atsushi Enomoto and Masahide Takahashi, Nagoya University in 2021. This strain was generated using CSL3 (ES cell) derived from 129S6/SvEvTac and mice were backcrossed to C57BL/6J for more than 8 generations.
Strain descriptionCre/ERT2 konckin mice in which an open reading frame of the Islr gene was replaced with Cre/ERT2.
Colony maintenance
ReferencesCancer Res., 79(20):5367-5381 (2019). doi: 10.1158/0008-5472.CAN-19-0454. 31439548

Health Report

Examination Date / Room / Rack2022/04/11Room:3-BRack:H


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Islrimmunoglobulin superfamily containing leucine-rich repeat9Islr<tm1.1(cre/ERT2)Mat>targeted mutation 1.1,Masahide TakahashiMeflin

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ERT2estrogen receptor (ER), human ER with the triple mutation G400V/M543A/L544A)9ERT2

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site9Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBBhuman beta-globin polyA9HBB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
crePhage P1 Cre recombinase9cre


Donor DNAP1 bacteriophage Cre, Human ERT2 cDNA, Human beta-globin polyA, Saccharomyces cerevisiae FRT site, Mouse Islr (knock-in)genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cancer Res. 2019 Oct 15;79(20):5367-5381. doi: 10.1158/0008-5472.CAN-19-0454.For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT shall reach an agreement with the DEPOSITOR on terms and conditions of use of the BIOLOGICAL RESOURCE and shall obtain a prior written consent from the DEPOSITOR. The RECIPIENT must contact the DEPOSITOR in the case of application for an patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAtsushi Enomoto (Nagoya University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data