Strain Data Sheet

RBRC11434

Strain Information

Image
BRC No.RBRC11434
TypeTargeted MutationCartagena
SpeciesMus musculus
Strain nameB6;129-Kdr<tm2.1Msh>
Former Common nameFLK1<1212F/+> mice, FLK1<1212F/1212F> mice
H-2 Haplotype
ES Cell lineE14 [129P2/OlaHsd]
Background strain
Appearance
Strain developmentDeposited by Masabumi Shibuya, Jobu University in 2021. This strain was generated using E14 (ES Cell) derived from 129P2/OlaHsd. Mice were backcrossed to C57BL.6 for several generations. Mixed genetic background.
Strain descriptionKdr mutant harboring a point mutation (Y1212F) in the Kdr gene. A neo gene was deleted by CAG-Cre cross.
Colony maintenance
References
Essential role of Flk-1 (VEGF receptor 2) tyrosine residue 1173 in vasculogenesis in mice.
Sakurai Y, Ohgimoto K, Kataoka Y, Yoshida N, Shibuya M
Proc. Natl. Acad. Sci. USA, 102, 1076-1081 (2005). 15644447

Myc-dependent endothelial proliferation is controlled by phosphotyrosine 1212 in VEGF receptor-2.
Testini C, Smith R O, Jin Y, Martinsson P, Sun Y, Hedlund M, Sainz-Jaspeado M, Shibuya M, Hellstrom M, Claesson-Welsh L
EMBO Rep. Nov 5;20(11):e47845 (2019). 31545012

Health Report

Examination Date / Room / Rack2025/10/20Room:4-CRack:JSentinel mouse program
2025/08/04Room:4-CRack:JSentinel mouse program
2025/05/26Room:4-CRack:JSentinel mouse program
2025/02/25Room:4-CRack:JSentinel mouse program
2024/11/25Room:4-CRack:JSentinel mouse program

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Kdr
MGI:96683		kinase insert domain protein receptor5Kdr<tm2.1Msh>
MGI:3530469		targeted mutation 2.1, Masabumi Shibuya
  • capillary infantile hemangioma(MedGEN)
    • loxP phage P1 loxP5loxP

    Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNAphage P1 loxP site, mouse FLK1 gene: Mutation in Exon-27 (including mutation at codon 1212)
    Research applicationCre/loxP system
    Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc. Natl. Acad. Sci. USA., 102, 1076-1081 (2005). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
    DepositorMasabumi Shibuya (Jobu University)
    Strain Statusan icon for Live miceLive mice
    an icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovery and QC required prior to distribution
    Cryopreserved sperm (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Order form (Japanese / English)
    2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
    3. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data