Strain Data Sheet

RBRC11432

Strain Information

Image
BRC No.RBRC11432
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Flt1<tm2Msh>
Former Common nameFLT1<TM-TK-/- > mice, FLT1<TM-TK+/-> mice
H-2 Haplotype
ES Cell lineCCE/EK.CCE [129S/SvEv-Gpi1<c>]
Background strain
Appearance
Strain developmentDeposited by Masabumi Shibuya, Jobu University in 2021. This strain was generated using CCE/EK.CCE (ES cell) derived from 129S/SvEv-Gpi1<c>. Mice were backcrossed to C57BL.6. Mixed genetic background.
Strain descriptionFlt1 mutant mice lacking kinase and transmembrane domain.
Colony maintenance
References
Membrane fixation of vascular endothelial growth factor receptor 1 ligand-binding domain is important for vasculogenesis and angiogenesis in mice.
Hiratsuka S, Nakao K, Nakamura K, Katsuki M, Maru Y, Shibuya M
Mol Cell Biol., 25(1):346-54 (2005). 15601855

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Flt1
MGI:95558		FMS-like tyrosine kinase 15Flt1<tm2Msh>
MGI:3527129		targeted mutation 2, Masabumi Shibuya
neo neomycin resistance gene (E. coli)5

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNAE. coli Neomycin-resistant gene, Mouse FLT1 gene: Intron-15, -16の一部、Exon-17とその下流（参考文献１、Figure 1参照）
Research application
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Mol Cell Biol., 25, 346-354 (2005).In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorMasabumi Shibuya (Jobu University)
Strain Statusan icon for Frozen embryosFrozen embryos
Strain AvailabilityRecovery and QC required prior to distribution
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

BRC mice in Publications

No Data