Strain Data Sheet
RBRC11431
Strain Information | |
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| Image | |
| BRC No. | RBRC11431 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6;Cg-Flt1<tm1Msh> |
| Former Common name | FLT1<TK-/-> mice, FLT1TK<+/-> mice |
| H-2 Haplotype | |
| ES Cell line | J1 [129S4/SvJae] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Masabumi Shibuya, Jobu University in 2021. This strain was generated using J1 (ES Cell) derived from 129S4/SvJae. Mice were backcrossed to C57BL.6 for several generations. Mixed genetic background. |
| Strain description | Flt1 mutant mice lacking kinase domain. |
| Colony maintenance | |
| References | Flt-1 lacking the tyrosine kinase domain is sufficient for normal development and angiogenesis in mice. Hiratsuka S, Minowa O, Kuno J, Noda T, Shibuya M Proc. Natl. Acad. Sci. USA, 95(16):9349-54 (1998). 9689083MMP9 induction by vascular endothelial growth factor receptor-1 is involved in lung-specific metastasis. Hiratsuka S, Nakamura K, Iwai S, Murakami M, Itoh T, Kijima H, Shipley J M, Senior R M, Shibuya M Cancer Cell, 2(4):289-300 (2002). 12398893Signaling of vascular endothelial growth factor receptor-1 tyrosine kinase promotes rheumatoid arthritis through activation of monocytes/macrophages. Murakami M, Iwai S, Hiratsuka S, Yamauchi M, Nakamura K, Iwakura Y, Shibuya M Blood, 108(6):1849-56 (2006). 16709927Vascular endothelial growth factor receptor-1 signaling promotes mobilization of macrophage lineage cells from bone marrow and stimulates solid tumor growth. Muramatsu M, Yamamoto S, Osawa T, Shibuya M Cancer Res., 70(20):8211-21 (2010). 20924106A Functional Role for VEGFR1 Expressed in Peripheral Sensory Neurons in Cancer Pain. Selvaraj D, Gangadharan V, Michalski C W, Kurejova M, Stosser S, Srivastava K, Schweizerhof M, Waltenberger J, Ferrara N, Heppenstall P, Shibuya M, Augustin H G, Kuner R Cancer Cell., 27(6):780-96 (2015). 26058077 |
Health Report | |
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| Examination Date / Room / Rack | 2025/10/20Room:4-CRack:JSentinel mouse program 2025/08/04Room:4-CRack:JSentinel mouse program 2025/05/26Room:4-CRack:JSentinel mouse program 2025/02/25Room:4-CRack:JSentinel mouse program 2024/11/25Room:4-CRack:JSentinel mouse program |
Gene | |||||||
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| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Flt1 MGI:95558 | FMS-like tyrosine kinase 1 | 5 | Flt1<tm1Msh> MGI:1934260 | targeted mutation 1, Masabumi Shibuya | |||
| neo | neomycin resistance gene (E. coli) | 5 | mouse Phosphoglycerate kinase (PGK) promoter | ||||
Phenotype | |
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| Phenotype annotation from literatures by Mammalian phenotype ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | mouse Phosphoglycerate kinase (PGK) promoter, E. coli Neomycin-resistant gene, Mouse FLT1 gene: Part of Intron-16, -17 genomic DNA |
| Research application | Mouse Models for Human Disease |
| Specific Term and Conditions | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Proc Natl Acad Sci USA., 95(16):9349-54 (1998). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. (from the Deposition time on 2021 year to 2023y.3.31. After that, there is no requirement for this item.) |
| Depositor | Masabumi Shibuya (Jobu University) |
| Strain Status |  Live mice Frozen embryos Frozen sperm |
| Strain Availability |  Recovery and QC required prior to distributionCryopreserved sperm (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |