Strain Data Sheet

RBRC11406

Information1

Image
BRC No.RBRC11406
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameB6.Cg-Klhl8<em1Tfur>
Former Common nameKlhl8 KO
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Takahisa Furukawa, Osaka University in 2021. This strain was generated using fertilized eggs derived from BDF1 and mice were further backcrossed to C57BL/6J for nine generations.
Strain descriptionKlhl8 mutant mice lacking 8 bp deletion at exon 2 which results in premature translation termination.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Klhl8kelch-like 85Klhl8<em1Tfur>endonuclease-mediated mutation 1, Takahisa Furukawa

Information2

Donor DNAStreptococcus pyogenes U6 promoter, Streptococcus pyogenes gRNA scaffold,human U6 terminator, CMV, chicken hybrid CMV enhancer/chicken beta-actin promotor (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal (NLS), Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal, AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka University.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data