Strain Data Sheet


Strain Information

BRC No.RBRC11404
SpeciesMus musculus
Strain nameB6.Cg-Herc3<em1Tfur>
Former Common nameHerc3 KO
H-2 Haplotype
ES Cell line
Background strain
Strain developmentDeposited by Takahisa Furukawa, Osaka University in 2021. This strain was generated using fertilized eggs derived from B6C3F1 and mice were further backcrossed to C57BL/6J for six generations.
Strain descriptionHerc3 mutant mice lacking 38 bp deletion at exon 2 which results in premature translation termination.
Colony maintenance

Health Report

Examination Date / Room / Rack


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Herc3hect domain and RLD 36Herc3<em1Tfur>endonuclease-mediated mutation 1, Takahisa Furukawa

Ordering Information

Donor DNAStreptococcus pyogenes U6 promoter, Streptococcus pyogenes gRNA scaffold, human U6 terminator, CMV, chicken hybrid CMV enhancer/chicken beta-actin promoter (CBh), Synthetic DNA 3xFLAG, SV40 nuclear localization signal (NLS)Streptococcus pyogenes SpCas9* (human codon-optimized), bovine GH polyA signal,AAV2 inverted terminal repeat (ITR), f1 phage f1 origin, E. coli Ampicillin resistance gene (AmpR), E. coli pUC origin* Not detected by PCR using Marker Gene Detection kit (TOYOBO, Osaka, Japan). Other introduced genes were not tested.
Research application
Specific Term and ConditionsIn publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The RECIPIENT must contact the DEPOSITOR and contract an MTA with Osaka University.
DepositorTakahisa Furukawa (Osaka University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data