Strain Data Sheet

RBRC11400

Strain Information

Image
BRC No.RBRC11400
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameC.Cg-Spib<tm2.1Ksho>
Former Common nameSpi-B flox BALB
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeveloped by Tsuneyasu Kaisho, Research Center for Allergy and Immunologuy, RIKEN around 2005. Backrossed to BALB/cAJcl by Koji Hase, Keio University Faculty of Pharmacy around 2019-2020.
Strain descriptionSpi-B is an Ets family transcription factor. In B6.Cg-Spib xxxx mice, two loxP sites are inserted in the introns of the Spi-B gene locus. Expression of cre recombinase leads to deletion of Spi-B coding exon(s) flanked by the loxP sites and disruption of the Spi-B gene locus. Spi-B-deficient mice show various phenotypes, such as defects in plasmacytoid dendritic cell function and development and defective development of an intestinal epithelial cell subset, M cell.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
Frt yeast FRT (flippase recombination target) site7Frt
Spib
MGI:892986		Spi-B transcription factor (Spi-1/PU.1 related)7Spib<tm2.1Ksho> targeted mutation 2.1, Tsuneyasu Kaisho
loxP phage P1 loxP7loxP
loxP phage P1 loxP7loxP

Phenotype

Phenotype annotation from literatures by Mammalian phenotype ontology
Detailed phenotype data

Ordering Information

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse Spib genomic DNA
Research applicationCre/loxP system
FLP/frt system
Immunology and Inflammation Research
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval on terms and conditions of use of it with the DEPOSITOR(Dr. Tsuneyasu Kaisho) and CO-DEVELOPER (Dr. Koii Hase).
DepositorTsuneyasu Kaisho (RIKEN)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data