Strain Data Sheet
RBRC11399
Strain Information | |
|---|---|
| Image | |
| BRC No. | RBRC11399 |
| Type | Targeted Mutation Congenic |
| Species | Mus musculus |
| Strain name | B6.Cg-Arntl<tm1Shbi> |
| Former Common name | Bmal1<flox>, B6.Cg-Arntl<tm1Shbi> |
| H-2 Haplotype | |
| ES Cell line | Bruce 4 [B6.Cg-Thy1<a>] |
| Background strain | |
| Appearance | |
| Strain development | Deposited by Shigeki Shinba, Nihon University in 2021. This strain was generated using Bruce 4 (ES cell) derived from B6.Cg-Thy1.1. |
| Strain description | Floxed Bmal1 (Arntl) mouse. A frt-flanked neo gene exists between exon 8 and 9. |
| Colony maintenance | |
| References | Deletion of Bmal1 Prevents Diet-Induced Ectopic Fat Accumulation by Controlling Oxidative Capacity in the Skeletal Muscle. Wada T, Ichihashi Y, Suzuki E, Kosuge Y, Ishige K, Uchiyama T, Makishima M, Nakao R, Oishi K, Shimba S Int. J. Mol. Sci., 19(9):2813 (2018). 30231537Deficient of a clock gene, brain and muscle Arnt-like protein-1 (BMAL1), induces dyslipidemia and ectopic fat formation. Shimba S, Ogawa T, Hitosugi S, Ichihashi Y, Nakadaira Y, Kobayashi M, Tezuka M, Kosuge Y, Ishige K, Ito Y, Komiyama K, Okamatsu-Ogura Y, Kimura K, Saito M PLoS One. 6(9):e25231 (2011). 21966465 |
Health Report | |
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| Examination Date / Room / Rack | 2023/02/13Room:3-CRack:K 2023/01/23Room:3-CRack:K |
Gene | |||||||
|---|---|---|---|---|---|---|---|
| Gene Symbol | Gene Name | Chr. | Allele Symbol | Allele Name | Common Names | Promoter | Diseases Related to This Gene |
| Bmal1 | basic helix-loop-helix ARNT like 1 | 7 | Bmal1 | endonuclease mediated mutation 1, Shigeki Simba | |||
| Frt | yeast FRT (flippase recombination target) site | 7 | Frt | ||||
| GH | Growth hormone polyA (Bovine) | 7 | GH | ||||
| loxP | phage P1 loxP | 7 | loxP | ||||
| loxP | phage P1 loxP | 7 | loxP | ||||
| neo | neomycin resistance gene (E. coli) | 7 | mouse phosphoglycerate kinase promoter (PGK promoter) | ||||
Phenotype | |
|---|---|
| Annotation by Mammalian phenotyhpe ontology | |
| Detailed phenotype data | |
Ordering Information | |
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| Donor DNA | phage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, bovine GH polyA, yeast mutated FRT (flippase recombination target) site, mouse Arntl genomic DNA |
| Research application | Cre/loxP system FLP/frt system |
| Specific Term and Conditions | Prior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. |
| Depositor | Shigeki Simba (Nihon University) |
| Strain Status |  Frozen embryos Frozen sperm |
| Strain Availability | Recovered litters from cryopreserved embryos (2 to 4 months) Cryopreserved sperm (within 1 month) Cryopreserved embryos (within 1 month) |
| Additional Info. | Necessary documents for ordering:
Genotyping protocol -PCR- |
BRC mice in Publications |
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| No Data |