Strain Data Sheet

RBRC11399

Information1

Image
BRC No.RBRC11399
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Arntl<tm1Shbi>
Former Common nameBmal1<flox>, B6.Cg-Arntl<tm1Shbi>
H-2 Haplotype
ES Cell lineBruce 4 [B6.Cg-Thy1<a>]
Background strain
Appearance
Strain developmentDeposited by Shigeki Shinba, Nihon University in 2021. This strain was generated using Bruce 4 (ES cell) derived from B6.Cg-Thy1.1.
Strain descriptionFloxed Bmal1 (Arntl) mouse. A frt-flanked neo gene exists between exon 8 and 9.
Colony maintenance
ReferencesInt. J. Mol. Sci., 19(9):2813 (2018). 30231537
PLoS One. 6(9):e25231 (2011). 21966465

Health Report

Examination Date / Room / Rack2023/02/13Room:3-CRack:K
2023/01/23Room:3-CRack:K
2022/10/25Room:3-CRack:K
2022/07/25Room:3-CRack:K

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Bmal1basic helix-loop-helix ARNT like 17Bmal1<tm1Shbi>endonuclease mediated mutation 1, Shigeki Simba

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
GHGrowth hormone polyA (Bovine)7GH

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
neoneomycin resistance gene (E. coli)7mouse phosphoglycerate kinase promoter (PGK promoter)

Information2

Donor DNAphage P1 loxP sites, yeast FRT (flippase recombination target) site, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neomycin resistance gene, bovine GH polyA, yeast mutated FRT (flippase recombination target) site, mouse Arntl genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form.
DepositorShigeki Simba (Nihon University)
Strain Statusan icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
Cryopreserved sperm (within 1 month)
Cryopreserved embryos (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data