Strain Data Sheet


Strain Information

BRC No.RBRC11393
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Ebf3<tm2.1Tng>
Former Common nameEbf3-flox
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Strain developmentDeposited by Takashi Nagasawa, Osaka University in 2021. This strain was generated using KY1.1 ES cells derived from 129/B6. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic line.
Strain descriptionEbf3-flox mouse. A pair of loxP flanks Exon 3 and Exon 4 of the Ebf3 gene.
Colony maintenance
ReferencesGenes Dev., 32, 359-372 (2018), 29563184

Health Report

Examination Date / Room / Rack2024/04/08Room:3-BRack:K


Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Ebf3early B cell factor 37Ebf3<tm2.1Tng>targeted mutation 2.1, Takashi Nagasawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Frtyeast FRT (flippase recombination target) site7Frt

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP7loxP

Ordering Information

Donor DNAP1 phage loxP sites, yeast FRT (flippase recombination target) site, mouse Ebf3 genomic DNA
Research applicationCre/loxP system
FLP/frt system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Genes Dev., 32, 359-372 (2018), RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takashi Nagasawa) and enter into a Material Transfer Agreement with Osaka University. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorTakashi Nagasawa (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (3 to 6 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data