Strain Data Sheet

RBRC11390

Information1

Image
BRC No.RBRC11390
TypeTargeted Mutation CongenicCartagena
SpeciesMus musculus
Strain nameB6.Cg-Cxcl12<tm4.1Tng>
Former Common nameCxcl12-flox
H-2 Haplotype
ES Cell lineKY1.1[(C57BL/6J x 129S6/SvEvTac)F1]
Background strain
Appearance
Strain developmentDeposited by Takashi Nagasawa, Osaka University in 2021. This strain was generated using KY1.1 ES cells derived from 129/B6. Mice were further backcrossed to C57BL/6J for 10 generations. C57BL/6J congenic line.
Strain descriptionCxcl12-flox mouse. Exon 2 of the Cxcl12 gene is floxed by a pair of loxP.
Colony maintenance
References

Health Report

Examination Date / Room / Rack2022/07/11Room:3-BRack:D
2022/04/11Room:3-BRack:D
2022/01/24Room:3-BRack:D

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Cxcl12chemokine (C-X-C motif) ligand 126Cxcl12<tm4.1Tng>targeted mutation 4.1, Takashi Nagasawa

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
loxPphage P1 loxP6loxP

Information2

Donor DNAP1 phage loxP sites, mouse Cxcl12 genomic DNA
Research applicationCre/loxP system
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. RECIPIENT which wants to use the BIOLOGICAL RESOURCE is requested to contact the depositor (Dr. Takashi Nagasawa) and enter into a Material Transfer Agreement with Osaka University. For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR.
DepositorTakashi Nagasawa (Osaka University)
Strain Statusan icon for Live miceLive mice
an icon for Frozen embryosFrozen embryos
an icon for Frozen spermFrozen sperm
Strain AvailabilityCryopreserved sperm (within 1 month)
Live mouse (1 to 3 months)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data