Strain Data Sheet

RBRC11387

Information1

Image
BRC No.RBRC11387
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6N-Pmepa1<em1Akt>
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Akikot Kukita, Saga University in 2021. Mice were generated using C57BL/6N. C57BL/6N genetic background.
Strain descriptionPmepa1 mutant mice generated by the CRISPR/Cas9. 1 bp insertion at exon 2 of the Pmepa1 gene.
Colony maintenance
ReferencesFASEB J., 35(2):e21281 (2021). 33484199
FASEB J., 33(3):4365-4375 (2019). 30557043

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Pmepa1prostate transmembrane protein, androgen induc2Pmepa1<em1Akt>

Information2

Donor DNA
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. FASEB J., 35(2):e21281 (2021).The RECIPIENT must contact the DEPOSITOR in the case of applications for any patents or commercial use base on the results from the use of the BIOLOGICAL RESOURCE.
DepositorAkiko Kukita (Saga University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data