Strain Data Sheet

RBRC11386

Information1

Image
BRC No.RBRC11386
TypeTransgene CongenicCartagena
SpeciesMus musculus
Strain nameB6;Cg-Tg(Ctsk-OCZF)#Akt
Former Common name
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Akiko Kukita, Saga University in 2021. This strain was generated using fertilized eggs derived from B6D2F1 and the mice were further backcrossed to C57BL/6N for three generations. Mixed genetic background.
Strain descriptionTransgenic mice expressing rat OCZF/LRF/FBI-1 under the control of mouse cathepsin K promoter.
Colony maintenance
ReferencesBlood, 94(6):1987-97 (1999). 10477728
Arthritis Rheum., 63(9):2744-54 (2011). 21590684

Health Report

Examination Date / Room / Racknull

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
EGFPEnhanced Green Fluorescent Protein (Aequorea victoria)UNEGFP

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HBBhuman beta-globinUNHBB

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Iresinternal ribosomal entry site (EMCV)UN

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
ZBTB7Azinc finger and BTB domain containing 7a (Rat)UNZBTB7AOCZFmouse Cathepsin K promoter

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
SV40 polyA signalUN

Information2

Donor DNAmouse Cathepsin K promoter, human 5'-donor site from the first intron of the human beta-globin gene and the branch and 3'-acceptor site from the intron of an immunoglobulin gene heavy chain variable region, rat OCZF cDNA, encephalomyocarditis virus, EMCV internal ribosomal entry site (IRES), Aequorea victoria enhanced green fluorescent protein (EGFP)cDNA, SV 40 poly A signal
Research applicationFluorescent Proteins/lacZ System
Specific Term and ConditionsIn publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Arthritis Rheum., 63(9):2744-54 (2011).
DepositorAkiko Kukita (Saga University)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Order form (Japanese / English)
  2. Category I MTA: MTA for distribution with RIKEN BRC (Japanese / English)
  3. Acceptance of responsibility for living modified organism (Japanese / English)
  4. GFP Transfer License (Japanese / English)
    Please fill in the Schedule A, and submit two signed copies to us together with two signed copies of RIKEN BRC's MTA. Please also read Schedule B.

Genotyping protocol -PCR-

BRC mice in Publications

No Data