Strain Data Sheet

RBRC11379

Strain Information

Image
BRC No.RBRC11379
TypeCRISPR/Cas9Cartagena
SpeciesMus musculus
Strain nameC57BL/6-F8<em1Tsuka>
Former Common nameF8 knockout mice(CRISPR/Cas9)
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeposited by Tsukasa Ohmori, Jichi Medical University in 2021. C57BL/6J genetic background.
Strain descriptionF8 mutant mice generated by the CRISPR/Cas9. 2 bp deletion at exon 14 of the F8 gene. Mutant mice have only 2% of factor VIII activity and tend to bleeding.
Colony maintenance
References

Health Report

Examination Date / Room / Rack

Gene

Gene SymbolGene NameChr.Allele SymbolAllele NameCommon NamesPromoterDiseases Related to This Gene
F8
MGI:88383		coagulation factor VIIIXF8<em1Tsuka> endonuclease-mediated mutation 1, Tsukasa Ohmori
  • hemophilia A(DisGeNET, MedGEN)

    • Phenotype

    Phenotype annotation from literatures by Mammalian phenotype ontology
    Detailed phenotype data

    Ordering Information

    Donor DNA
    Research applicationMouse Models for Human Disease
    Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. The RECIPIENT agrees to use this BIOLOGICAL RESOURCE as a collaboration with the DEPOSITOR.
    DepositorTsukasa Ohmori (Jichi Medical University)
    Strain Statusan icon for Frozen embryosFrozen embryos
    an icon for Frozen spermFrozen sperm
    Strain AvailabilityRecovered litters from cryopreserved embryos (2 to 4 months)
    Cryopreserved sperm (within 1 month)
    Cryopreserved embryos (within 1 month)
    Additional Info.Necessary documents for ordering:
    1. Approval form (Japanese / English)
    2. Order form (Japanese / English)
    3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
    4. Acceptance of responsibility for living modified organism (Japanese / English)

    Genotyping protocol -PCR-

    BRC mice in Publications

    No Data