Strain Data Sheet

RBRC11377

Information1

Image
BRC No.RBRC11377
TypeCRISPR/Cas9 (Transgene)Cartagena
SpeciesMus musculus
Strain nameC57BL/6-Atf5<em1(Atf5/HA)Yuta>
Former Common nameATF5-HA knock-in, B6-ATF5HA KI
H-2 Haplotype
ES Cell line
Background strain
Appearance
Strain developmentDeveloped by Haruo Nakano, Tokyo university of the pharmacy and life sciences in 2019.The HA-tag was introduced into zygotes by CRISPR/Cas9 genome editing. C57BL/6NJcl (CLEA Japan Inc.)The ATF5-HA knock-in male G0 offspring was mated with wild type females, and then propageted by sibling crossing (Heterozygous x Heterozygous or Homozygous x Homozygous).
Strain descriptionATF5-Hemagglutinin (HA) knock-in mice. A HA-tag sequence was integrated into the stop codon site of ATF5 gene at an in-flame position.
Colony maintenance
ReferencesCell Tissue Res., (2021). doi: 10.1007/s00441-021-03450-7. 33825962

Health Report

Examination Date / Room / Rack

Gene

Gene info
Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
Atf5activating transcription factor 57Atf5<em1(Atf5/HA)Yuta>endonuclease-mediated mutation 1, Yuji Takahashi

Gene symbolGene nameChr.Allele symbolAllele nameCommon namesPromoter
HAhuman influenza virus HA (hemagglutinin) tag sequence7HA

Information2

Donor DNAinfluenza virus HA (hemagglutinin) tag sequence
Research application
Specific Term and ConditionsPrior to requesting the BIOLOGICAL RESOURCE, the RECIPIENT must obtain approval from the DEPOSITOR using the Approval Form. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Cell Tissue Res. 2021 Apr 7. doi: 10.1007/s00441-021-03450-7.In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
DepositorYuji Takahashi (Tokyo University of Pharmacy and Life Sciences)
Strain Statusan icon for Frozen spermFrozen sperm
Strain AvailabilityRecovered litters from cryopreserved sperm (2 to 4 months)
Cryopreserved sperm (within 1 month)
Additional Info.Necessary documents for ordering:
  1. Approval form (Japanese / English)
  2. Order form (Japanese / English)
  3. Category I MTA: CRISPR/Cas9 genome edited bioresources (Japanese / English)
  4. Acceptance of responsibility for living modified organism (Japanese / English)

Genotyping protocol -PCR-

BRC mice in Publications

No Data